Abstract: Objective To determine the knowledge on and discriminative attitudes towards HIV/AIDS among health workers, in order to identify the influential factors of the development of discriminative attitudes.Methods An anonymous questionnaire-based quota sampling was utilized to collect the information including demographics, knowledge and discriminative attitudes related to HIV/AIDS.Results A total of 7504 medical workers were surveyed.As a result, the knowledge associated with HIV/AIDS was not comprehensively adequate.On the contrary, the awareness of HIV/AIDS was relatively higher in the center for disease control.25.1% of medical workers presented discriminative attitudes towards HIV/AIDS patients.On the other hand, medical workers with higher awareness of HIV/AIDS, those from CDC, the married or cohabitants, and those older than 25 years old had less discrimination.Conclusion Public health workersAIDS-related should be further improved.Deficiency in knowledge of HIV/AIDS constituted the major reason for discrimination, thus justifying further training for medical staff.